index .. maintenance .. molecular .. histology .. immunocyt .. morpho & behav .. field .. accessories

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index of methods

maintenance
Macrostomum cultures
algae cultures
media

molecular

histology

immunocytochemistry

morphometry & behaviour

field sampling

accessories

^a philosophical algae^

Dita B. Vizoso

Email:
dita.vizoso_at_unibas.ch

Address:
Institute of Zoology
Evolutionary Biology
University of Basel
Vesalgassesse 1
4051 Basel
Switzerland

> the schärer group

Here there will be a series of protocols for histological work with Macrostomum. If you any protocols for fixation, sectioning (semi-thin, ultra-thin, serial, etc), analysing, ultrastructure, etc. please send them to me.

Serial Sections
A lot of histology and the discovery of the internal morphology of Macrostomida can be done with serial sections.

Here you find a mixture of methods compiled from tips from different people and our own trials.

Click on the links to download a pdf file.

protocol		used for
embedding with Spurr		serial sectioning
Download PDF (96 KB) The dehydration, infiltration and embedding procedures as performed in Basel. Protocol optimized by Dita Vizoso, from various sources.

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last uptaded: 07.06.2010