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Culturing Daphnia food

Please cite as: Ebert, D. Web-guide to Daphnia parasites.

Chemostats and pumps

Five chemostats with peristaltic pumps in the foreground and feeding bottles in the background

Green algae Scenedesmus

Green algae Scenedesmus

Scenedesmus in a counting chamber

Scendesmus in a counting chamber

We grow the green algae Scenedesmus in chemostats. The chemostats have a capacity of about 2.5 liters and a flowthrough of 2 liters per day. We use the following medium, which is routinely produced by mixing 7 stock solutions with TES in deionized (or destilled) water. To produce 20 liter algae medium add to 20 liter water: 2.3 g TES and 20 ml of each of the 7 stock solutions A - G (in this order!). Afterwards we pump the entire medium through a 0.2 micro-meter filter to sterilize it. (Alternative sterilize in autoclave)

Stock solution Chemical Concentration [g/l]
A CaCl2× 2 H20 36.8
B MgSO4× 7 H2O 37.0
C NaHCO3 12.6
D K2HPO4× 3 H2O 11.4
E NaNO3 85.0
F Na2SiO3× 5 H2O 21.2
G* NaEDTA 4.360
FeCl3× 6 H2O 3.150
CuSO4× 5 H2O 0.010
ZnSO4× 7 H2O 0.022
CoCl2× 6 H2O 0.010
MnCl2× 4 H2O 0.180
Na2MoO4× 2 H2O 0.006
H3BO3 1.000

* Solution G contains among others, all the trace elements.

Some notes on how we run the chemostats:

Chemostats and feeding bottles

Full set of chemostats for algae mass production