Please cite as: Ebert, D. Web-guide to Daphnia parasites.
Five chemostats with peristaltic pumps in the foreground and feeding bottles in the background
Green algae Scenedesmus
Scendesmus in a counting chamber
We grow the green algae Scenedesmus in chemostats. The chemostats have a capacity of about 2.5 liters and a flowthrough of 2 liters per day. We use the following medium, which is routinely produced by mixing 7 stock solutions with TES in deionized (or destilled) water. To produce 20 liter algae medium add to 20 liter water: 2.3 g TES and 20 ml of each of the 7 stock solutions A - G (in this order!). Afterwards we pump the entire medium through a 0.2 micro-meter filter to sterilize it. (Alternative sterilize in autoclave)
| Stock solution | Chemical | Concentration [g/l] |
|---|---|---|
| A | CaCl2× 2 H20 | 36.8 |
| B | MgSO4× 7 H2O | 37.0 |
| C | NaHCO3 | 12.6 |
| D | K2HPO4× 3 H2O | 11.4 |
| E | NaNO3 | 85.0 |
| F | Na2SiO3× 5 H2O | 21.2 |
| G* | NaEDTA | 4.360 |
| FeCl3× 6 H2O | 3.150 | |
| CuSO4× 5 H2O | 0.010 | |
| ZnSO4× 7 H2O | 0.022 | |
| CoCl2× 6 H2O | 0.010 | |
| MnCl2× 4 H2O | 0.180 | |
| Na2MoO4× 2 H2O | 0.006 | |
| H3BO3 | 1.000 |
* Solution G contains among others, all the trace elements.
Some notes on how we run the chemostats: