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Artificial Daphnia medium: ADaM (Aachener Daphnien Medium)

We grow Daphnia in artificial medium. The original recipe for the medium was developed by Klüttgen, B., Dülmer U., Engels, M. & Ratte H. T. 1994. ADaM, an artificial freshwater for the culture of zooplankton. Water Research 28:743-746. We use a modified version of this medium (altered Seleniumdioxid concentration). To produce a given amount of medium add the amounts of sea salt and stock solutions as given in the following table.

Container with ADaM

60 l medium tank in our lab

Water Sea salt [gram] Stock solution A (ml) Stock solution B (ml) Stock solution C (ml)
10 l 3.33 23 22 1
50 l 16.6 115 110 5
60 l 19.9 138 132 6

We use a special sea salt which is produced by combining all chemical to make up the mixture. (Most commercially available sea salt is produced by evaporation of water from sea water. In this case, some of the salts do not solve anymore once put back into water). We buy this sea salt from an aquarium supply company. Atrificial sea salt works fine.

These are the concentrations for the three stock solutions:

Stock solution Chemical Concentration [g/l]
A CaCl2 x 2H2O 117.6
B NaHCO3 25.2
C SeO2 0.07

Some notes on our experience with this medium: